The Inhibition of Superoxide Production by the Soluble Phase of Cystic Fibrosis Sputum



Amrita Dosanjh*
Department of Pediatrics, University of California School of Medicine, San Diego, CA 92122, USA


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© Amrita Dosanjh; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Pediatrics, University of California School of Medicine, San Diego, CA 92122, USA; Tel: 858 442 6146; E-mail: adosanjh@ucsd.edu


Abstract

Background

Cystic fibrosis (CF) lung disease is characterized by an infiltration of activated neutrophils. Oxidative radical formation and neutrophil proteases may interact with the components of CF airway secretions. This study examined the soluble factors in CF airway secretions (sol) and determined their effect on the production of superoxide radicals.

Methods

Neutrophils were isolated from three normal subjects. Each preparation of neutrophils can be used in several experiments on the same day of preparation. Using a standardized cytochrome c reduction method, the effect of pooled CF sol isolated from nine CF patients, on neutrophil superoxide production was studied.

Results

CF sol phase significantly attenuates the production of superoxide by phorbol stimulated control neutrophils in a time and concentration dependent manner. When CF sol is boiled, the inhibitory effect on superoxide was reversed. CF soluble heat labile factors clearly attenuated superoxide production, independent of elastase concentration. We conclude that the presence of heat labile soluble factors in CF sputum, serve to attenuate superoxide production by human neutrophils.

Keywords: Cystic fibrosis, superoxide, neutrophil, sputum..