RESEARCH ARTICLE
Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias
Martina Vasakova*, 1, Martina Sterclova 1, Eliska Stranska 2, Petra Mandakova 2, Jelena Skibova 3, Radoslav Matej 4
Article Information
Identifiers and Pagination:
Year: 2012Volume: 6
First Page: 160
Last Page: 164
Publisher ID: TORMJ-6-160
DOI: 10.2174/1874306401206010160
Article History:
Received Date: 10/10/2012Revision Received Date: 11/11/2012
Acceptance Date: 21/11/2012
Electronic publication date: 28/12/2012
Collection year: 2012

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Abstract
Aims:
The main feature of fibrosing idiopathic interstitial pneumonias (fIIPs) is the fibroproliferative potential of underlying pathogenetic process. We hypothesize that the concentration of potential markers of fibroproliferative healing, PAR-2, TGF-β1, TNF-α and IL-4Rα in bronchoalveolar lavage fluid (BALF) differ in patients with fIIPs compared to controls (C).
Patients and Methods:
10 patients with fIIPs and 9 controls (C) were included to the study.
Concentrations of CD124 (IL4Rα), PAR-2, TGF-β1 and TNF-α in BALF were determined using the ELISA method.
Results:
We observed higher concentrations of IL4Rα (fIIPS 1499.4 pg/ml vs C 255.5 pg/ml; p < 0.05), PAR-2 (fIIPS 1807.9 pg/ml vs C 421.0 pg/ml; p < 0.05) and TGF-β1(fIIPS 283.0 pg/ml vs C 197.1 pg/ml; p < 0.01) in BALF in fIIPs versus C. The values of TNF-a in BALF did not differ significantly in fIIPs compared to controls. The ratios also showed differences in fIIPS and C: IL4Rα/TGF-β1 (fIIPS 6.19 vs C 0.68; p = 0.0143); TNF-α/IL4Rα (fIIPS 0.84 vs C 7.93; p = 0.043); TGF-β1/TNF-α (fIIPS 0.21 vs C 0.16; p = 0.0179) and protein/PAR-2 (fIIPS 0.06 vs C 0.28; p = 0.0033).
Conclusions:
We found that PAR-2, TGF-β1 and IL-4Rα are significantly up-regulated in the BALF of fIIPs compared to controls, therefore we suppose they could become biomarkers of fibroproliferative healing.