Biomarkers of Fibroproliferative Healing in Fibrosing Idiopathic Interstitial Pneumonias



Martina Vasakova*, 1, Martina Sterclova 1, Eliska Stranska 2, Petra Mandakova 2, Jelena Skibova 3, Radoslav Matej 4
1 Department of Respiratory Medicine, Thomayer Hospital, Videnska 800, 14059 Prague 4, Czech Republic
2 Department of Pathology and Molecular Medicine, Charles University in Prague, 2nd Medical Faculty, V Uvalu 84, 15006 Prague 5, Czech Republic
3 Medical Statistics Unit, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 14021 Prague 4, Czech Republic
4 Department of Pathology and Molecular Medicine, Thomayer Hospital, Videnska 800, 14059 Prague 4, Czech Republic


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© Vasakova et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Respiratory Medicine, Thomayer Hospital Prague, Videnska 800, 140 59 Prague 4, Czech Republic; Tel: + 42026108 2372; Fax: + 42026108 2206; E-mail: martina.vasakova@ftn.cz


Abstract

Aims:

The main feature of fibrosing idiopathic interstitial pneumonias (fIIPs) is the fibroproliferative potential of underlying pathogenetic process. We hypothesize that the concentration of potential markers of fibroproliferative healing, PAR-2, TGF-β1, TNF-α and IL-4Rα in bronchoalveolar lavage fluid (BALF) differ in patients with fIIPs compared to controls (C).

Patients and Methods:

10 patients with fIIPs and 9 controls (C) were included to the study.

Concentrations of CD124 (IL4Rα), PAR-2, TGF-β1 and TNF-α in BALF were determined using the ELISA method.

Results:

We observed higher concentrations of IL4Rα (fIIPS 1499.4 pg/ml vs C 255.5 pg/ml; p < 0.05), PAR-2 (fIIPS 1807.9 pg/ml vs C 421.0 pg/ml; p < 0.05) and TGF-β1(fIIPS 283.0 pg/ml vs C 197.1 pg/ml; p < 0.01) in BALF in fIIPs versus C. The values of TNF-a in BALF did not differ significantly in fIIPs compared to controls. The ratios also showed differences in fIIPS and C: IL4Rα/TGF-β1 (fIIPS 6.19 vs C 0.68; p = 0.0143); TNF-α/IL4Rα (fIIPS 0.84 vs C 7.93; p = 0.043); TGF-β1/TNF-α (fIIPS 0.21 vs C 0.16; p = 0.0179) and protein/PAR-2 (fIIPS 0.06 vs C 0.28; p = 0.0033).

Conclusions:

We found that PAR-2, TGF-β1 and IL-4Rα are significantly up-regulated in the BALF of fIIPs compared to controls, therefore we suppose they could become biomarkers of fibroproliferative healing.

Keywords: Idiopathic interstitial pneumonias, fibroproliferative healing, biomarkers, bronchoalveolar lavage fluid, PAR-2, TGF-beta, IL4R..